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primary rabbit antihuman antibodies against twist1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary rabbit antihuman antibodies against twist1
    Figure 4. <t>TWIST1</t> was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.
    Primary Rabbit Antihuman Antibodies Against Twist1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary rabbit antihuman antibodies against twist1/product/Cell Signaling Technology Inc
    Average 93 stars, based on 45 article reviews
    primary rabbit antihuman antibodies against twist1 - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p."

    Article Title: CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p.

    Journal: Bioengineered

    doi: 10.1080/21655979.2021.1948487

    Figure 4. TWIST1 was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.
    Figure Legend Snippet: Figure 4. TWIST1 was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.

    Techniques Used: Binding Assay, Expressing, Quantitative RT-PCR, Transfection, Luciferase, Reporter Assay, Activity Assay, Western Blot

    Figure 5. miR-580-3p inhibitor or TWIST1 overexpression reversed effects of sh-circRAB3IP. MG63 cells were transfected with the control sh RNA (sh-NC), sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor or sh-circRAB3IP+TWIST1 overexpression vector (sh- circRAB3IP+oeTWIST1). (a, b) Cell proliferation was detected by CCK-8 assay and colony formation assay. (c, d) Transwell assay was employed to detect cell migration and invasion of OS cells. *P < 0.05.
    Figure Legend Snippet: Figure 5. miR-580-3p inhibitor or TWIST1 overexpression reversed effects of sh-circRAB3IP. MG63 cells were transfected with the control sh RNA (sh-NC), sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor or sh-circRAB3IP+TWIST1 overexpression vector (sh- circRAB3IP+oeTWIST1). (a, b) Cell proliferation was detected by CCK-8 assay and colony formation assay. (c, d) Transwell assay was employed to detect cell migration and invasion of OS cells. *P < 0.05.

    Techniques Used: Over Expression, Transfection, Control, Plasmid Preparation, CCK-8 Assay, Colony Assay, Transwell Assay, Migration



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    primary rabbit antihuman antibodies against twist1  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc primary rabbit antihuman antibodies against twist1
    Figure 4. <t>TWIST1</t> was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.
    Primary Rabbit Antihuman Antibodies Against Twist1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary rabbit antihuman antibodies against twist1/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    primary rabbit antihuman antibodies against twist1 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

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    Figure 4. TWIST1 was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.

    Journal: Bioengineered

    Article Title: CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p.

    doi: 10.1080/21655979.2021.1948487

    Figure Lengend Snippet: Figure 4. TWIST1 was targeted by miR-580-3p in OS (a) Bioinformatics was used to predict the potential binding site between miR- 580-3p and TWIST1. (b) TWIST1 was a target gene of miR-580-3p. (c, d) miR-580-3p mimics inhibited TWIST1 expression. (e) qRT-PCR indicated that TWIST1 was up-regulated in OS tissues. (f) Pearson’s analysis showed that circRAB3IP expression was positively correlated with TWIST1 expression in OS tissues. OS cells were transfected with sh-NC, sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor. (g) Luciferase reporter assay was used to determined the activity of TWIST1-Wt reporter. (h) TWIST1 expression was detected by Western blot. *P < 0.05.

    Article Snippet: Primary rabbit antihuman antibodies against TWIST1 (1:1000, cell signaling technology, #46702) and GAPDH (1:2000, Abcam, ab9485) were incubated overnight at 4°C.

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Transfection, Luciferase, Reporter Assay, Activity Assay, Western Blot

    Figure 5. miR-580-3p inhibitor or TWIST1 overexpression reversed effects of sh-circRAB3IP. MG63 cells were transfected with the control sh RNA (sh-NC), sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor or sh-circRAB3IP+TWIST1 overexpression vector (sh- circRAB3IP+oeTWIST1). (a, b) Cell proliferation was detected by CCK-8 assay and colony formation assay. (c, d) Transwell assay was employed to detect cell migration and invasion of OS cells. *P < 0.05.

    Journal: Bioengineered

    Article Title: CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p.

    doi: 10.1080/21655979.2021.1948487

    Figure Lengend Snippet: Figure 5. miR-580-3p inhibitor or TWIST1 overexpression reversed effects of sh-circRAB3IP. MG63 cells were transfected with the control sh RNA (sh-NC), sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor or sh-circRAB3IP+TWIST1 overexpression vector (sh- circRAB3IP+oeTWIST1). (a, b) Cell proliferation was detected by CCK-8 assay and colony formation assay. (c, d) Transwell assay was employed to detect cell migration and invasion of OS cells. *P < 0.05.

    Article Snippet: Primary rabbit antihuman antibodies against TWIST1 (1:1000, cell signaling technology, #46702) and GAPDH (1:2000, Abcam, ab9485) were incubated overnight at 4°C.

    Techniques: Over Expression, Transfection, Control, Plasmid Preparation, CCK-8 Assay, Colony Assay, Transwell Assay, Migration